AAV Purification Midi Kit – 63300

Recombinant adeno-associated virus (AAV) vectors are highly promising tools for bothin vitroandin vivogene transfer. Norgen’s AAV purification kit provides a fast and simple procedure for concentrating and purifying AAV vectors from cell lysate and cell culture media. Purification is based on spin column chromatography using Norgen’s proprietary resin as an ion exchanger. Contaminating cellular debris is largely removed from the sample via a precipitation and centrifugation step, while contaminating DNA and RNA is reduced using enzymatic digestion. AAV vector purified in this manner is highly active for use inin vitrotransduction experiments, and is eluted into a small volume (1 mL).